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测序通用引物全搜集

真·科研狗      10-17 10:43      2164      1

相信大家在送DNA测序的时候一般都要求填写载体的名称,如果是T载体或pcDNA3这种常见载体当然没有问题。但如果不是常用的载体,就需要自己合成引物(需要填写序列)或者指定一个通用引物的名称。实际上,我们在遇到后面一种情况时,可以分析下自己载体上有没有下面列举的常用元件。如果有,可以比较下后面列出的序列是否与你的载体序列匹配(这个是以防万一名称相同但序列却有差异),如果也是匹配的,就可以写上这些通用引物的名称(最好在下面也附上相应的序列,防止有些时候测序公司的通用引物库不全)。这样既可以节约自己合成引物的花费,而且这些引物的序列都是经反复优化的,可能比我们自己设计的序列效果更好!

下面是我们从网上搜集的最全的测序通用引物列表:


测序通用引物列表
引物名称
序列(5'→3')
描述
3'AOX1
GCAAATGGCATTCTGACATCC
For Pichia vectors with AOX1 terminator, reverse primer
5'AOX1
GACTGGTTCCAATTGACAAGC
For Pichia vectors with AOX1 promoter, forward primer
Polyhedrin forward
AAATGATAACCATCTCGC
Polyhedrin promoter, forward primer
pGP704-R
AACAAGCCAGGGATGTAACG
R6K gamma origin, 3' of MCS in pGP704 vector, reverse primer
SeqL-B (ATTL2)
AACATCAGAGATTTTGAGACAC
BAC1
AACCATCTCGCAAATAAATA
PIRES3-EGFP-R
AACGCACACCGGCCTTATTC
Ubx-F
AACTCGTACTTTGAACAGGC
Drosophila Ultrabithorax gene, forward primer
PBV220F
AAGAAGGGCAGCATTCAAAG
pbd-gal4-r
AAGAGTTACTCAAGAACAAGAA
pMRB101-F
AAGATGCAGGCAGCTGAGTT
HCMV major immediate-early protein (IE), forward primer
PB42AD-R
AAGCCGACAACCTTGATTGGAG
T7EEV
AAGGCTAGAGTACTTAATACG
Gal4-AD
AATACCACTACAATGGAT
3' end of Gal4 activation domain, forward primer
GAL4-ADfor
AATACCACTACAATGGATGATG
3' end of Gal4 activation domain, forward primer
pBRforEco
AATAGGCGTATCACGAGGC
In pBR322, upsteam of EcoRI site, forward primer
GAL1
AATATACCTCTATACTTTAACGTC
S. cerevisiae GAL1 promoter, forward primer
NOSR
AATCATCGCAAGACCGGC
GW-5'
AATCTCGCCGGATCCTAACT
5' end of Gateway cassette, reverse primer
AC5
ACACAAAGCCGCTCCATCAG
Drosophila Actin 5C promoter, forward primer
T7(17base)
ACATCCACTTTGCCTTTCTC
RCAS-F
ACATGGGTGGTGGTATAGCGCTTGCG
3' of Rous sarcoma virus (RSV) env gene, forward primer
GST-Tag
ACCCAATGTGCCTGGATGCG
pBABE 3'
ACCCTAACTGACACACATTCC
SV40 enhancer, 3' of MCS in pBABE vectors, reverse primer
V5 Reverse
ACCGAGGAGAGGGTTAGGGAT
V5 epitope, reverse primer
pLNCX-R
ACCTACAGGTGGGGTCTTTCATTCCC
BAC2
ACGCACAGAATCTAGCGCTT
pLTet-F
ACTGAGCACATCAGCAGGAC
Lambda phage early leftward (pL) promoter, forward primer
pCEP-F
AGAGCTCGTTTAGTGAACCG
F27
AGAGTTTGATCCTGGCTCA
3'AD
AGATGGTGCACGATGCACAG
L4440
AGCGAGTCAGTGAGCGAG
5' of MCS in L4440 vector, forward primer
M13 Reverse  (-48)
AGCGGATAACAATTTCACAC
M13/pUC Reverse
AGCGGATAACAATTTCACACAGG
In lacZ gene
M13R(-48)
AGCGGATAACAATTTCACACAGGA
In lacZ gene
LNCX
AGCTCGTTTAGTGAACCGTCAGATC
Human CMV promoter, forward primer
pLNCX-F
AGCTCGTTTAGTGAACCGTCAGATCG
DsRed1-C
AGCTGGACATCACCTCCCACAACG
3' end of DsRed1, forward primer
2.0rev primer
AGGCGATTAAGTTGGGTA
CMV-Reverse
AGTAGGAAAGTCCCGTAAGG
Luc-F
AGTCAAGTAACAACCGCGA
3' end of luciferase, forward primer
N-CMV-30
ATAACCCCGCCCCGTTG
Amp-R
ATAATACCGCGCCACATAGC
5' end of ampicillin resistance gene, reverse primer
MMLV-F
ATCAGTTCGCTTCTCGCTTC
Moloney murine leukemia virus LTR (MoMuLV), forward primer
pBAD Forward
ATGCCATAGCATTTTTATCC
For vectors with E. coli araBAD promoter, forward primer
pET-Upstream
ATGCGTCCGGCGTAGA
U6
ATGGACTATCATATGCTTACCGTA
pENTR-R
ATGGCTCATAACACCCCTTG
3' of attL2 in pENTR vector, reverse primer
T3
ATTAACCCTCACTAAAGGGA
T3 promoter, forward primer
pCMV5R
ATTATAGAGGACACCTAGTC
pDsRed-C-3'
ATTCATTTTATGTTTCAGGTTCA
SFFV-F
ATTGATTGACTGCCCACCTC
Spleen focus forming virus 5' LTR, forward primer
SP6
ATTTAGGTGACACTATAG
SP6 promoter, forward primer
S1
CAACGTGAAAAAATTATTATTCGC
TRC-F
CAAGGCTGTTAGAGAGATAATTGGA
Human U6 promoter, forward primer
T3
CAATTAACCCTCACTAAAGG
AUG1 Forward
CAATTTACATCTTTATTTATTAACG
For Pichia vectors with AUG1 promoter, forward primer
SV40-spliceR
CACAAAGATCCGGACCAAAG
SV40 splice sequence, reverse primer
pIRES.R
CACTGCATTCTAGTTGTGGT
mU6-F
CAGCACAAAAGGAAACTCACC
Mouse U6 promoter, forward primer
MSCV-rev
CAGCGGGGCTGCTAAAGCGCATGC
Murine stem cell virus, reverse primer
M13 Reverse
CAGGAAACAGCTATGAC
In lacZ gene
M13R
CAGGAAACAGCTATGACC
mU6
CAGTGCAGGGGAAAGAATAGTAGAC
WPRE-R
CATAGCGTAAAAGGAGCAACA
5' end of WPRE, reverse primer
MT Forward
CATCTCAGTGCAACTAAA
Drosophila metallothionein promoter, forward primer
EGFP-C
CATGGTCCTGCTGGAGTTCGTG
3' end of EGFP, forward primer
mPGK-F
CATTCTGCACGCTTCAAAAG
Mouse PGK promoter, forward primer
pTRE 3'
CCACACCTCCCCCTGAAC
PB42AD-F
CCAGCCTCTTGCTGAGTGGAGATG
hGH-PA-R
CCAGCTTGGTTCCCAATAGA
Human growth hormone terminator, reverse primer
Rluc-F
CCAGGATTCTTTTCCAATGC
3' end of Renilla luciferase, forward primer
pTarget.F
CCAGGATTTTCCCAGTCAC
pCAMBIA1301-5'
CCAGGCTTTACACTTTATGC
GFP-R
CCATCTAATTCAACAAGAATTGGGACAAC
5' end of GFP, reverse primer
M13/pUC Forward
CCCAGTCACGACGTTGTAAAACG
In lacZ gene
mCherry-F
CCCCGTAATGCAGAAGAAGA
3' end of mCherry, forward primer
pQE promoter
CCCGAAAAGTGCCACCTG
5' of MCS in pQE vectors, forward primer
Intein Forward
CCCGCCGCTGCTTTTGCACGTGAG
M13(-96)
CCCTCATAGTTAGCGTAACG

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