各位大神,这个问题如何回复?

智能科研狗 2016-09-05 17:51:28 阅读: 806

15DLBL:“In your previous query response 'The 3'-UTRs of CCND1 were amplified by PCR using the following primers: 5'-GTACTGGAGAATGTGCCCTGCGGCA-3' and 5'-AGCTCGRGTAAGGCTCTGCACCCGC-3’, an R has been included in the second primer sequence, please clarify what letter this should be replaced with. Please also provide details of the “appropriate primers" used to generate target site mutations.”


唐琼卫:R不就代表A/G么? Y.M.R都有特定的指代,很多文献都有这种表述方式。在合成引物的时候,合成公司都会提供简并碱基的对应表。如下:


小黑:“appropriate primers”,适当的引物,科研论文中不能出现这些模棱两可的词语,比如“一定的浓度”(到底是多少浓度),“基本有效”(有效率百分之多少)等。

 
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