pGL4.20

创建日期:2014-12-11 查看:96
  • 出品公司 Promega
  • 其他名称
  • 载体类型 哺乳细胞表达
  • 表达水平
  • 克隆方法 多克隆位点,限制性内切酶
  • 载体大小 5404bp
  • 5'端测序 --
  • 3'端测序 --
  • 载体标签 --
  • 载体抗性 氨苄青霉素(Ampicillin)
  • 稳定性 --
  • 组成 组成型
  • 病毒/非病毒 非病毒
GenBank:	DQ188840

LOCUS       pGL4.20[luc2/Puro]	5404 bp 	DNA	SYN
DEFINITION  pGL4.20[luc2/Puro]
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="pGL4.20[luc2/Puro]"
                     /mol_type="other DNA"
     CDS             100..1752
                     /label="ORF frame 1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     terminator      complement(1793..1912)
                     /label="SV40_PA_terminator"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     misc_feature    complement(1807..1826)
                     /label="EBV_rev_primer"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     misc_feature    complement(2134..2154)
                     /label="pBABE_3_primer"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     misc_feature    complement(2140..2355)
                     /label="SV40_enhancer"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     promoter        2152..2420
                     /label="SV40_promoter"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     rep_origin      2319..2396
                     /label="SV40_origin"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     misc_feature    2381..2400
                     /label="SV40pro_F_primer"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     CDS             complement(2454..3116)
                     /label="ORF frame 3"
                     /translation="MTGSRSLGARFSCHTPGPRSLGHLDIGGHGEAQALVEWKVSWCA
                     CFEKGGHTGAFGGLYAGQHDGAAQALALVVGAHSYSGQKPGRLLGTMGGKQALHLLLG
                     GQAAAAQLGHSGADLGEHGAGLNALGGSPHGHGRAVVGYPHLADVQPDAGQEQLLQLC
                     HTLDVPVGVHGVPGSGVVGESGGQSADGAGHIIASGQAHGRLVLGHGGASVRRIELFA
                     KA*"
     CDS             2499..3098
                     /label="ORF frame 3"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     terminator      3123..3171
                     /label="synth_PA_terminator"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     rep_origin      complement(3483..4102)
                     /label="pBR322_origin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     CDS             complement(4286..5146)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      5251..5404
                     /label="up_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      5251..5299
                     /label="synth_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5251..5404
                     /label="TB"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5353..5372
                     /label="RVprimer3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
   61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT
  121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC
  181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC
  241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG
  301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG
  361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC
  421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC
  481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA
  541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC
  601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC
  661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC
  721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC
  781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC
  841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC
  901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG
  961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC
 1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG
 1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC
 1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC
 1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC
 1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT
 1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC
 1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC
 1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC
 1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG
 1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA
 1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC
 1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG
 1741 ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA
 1801 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
 1861 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
 1921 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
 1981 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG
 2041 CTCTTCCGCT GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT
 2101 TAGCTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA
 2161 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA
 2221 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA
 2281 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA
 2341 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG
 2401 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT
 2461 TTTGCAAAAA GCTCGATTCT TCTGACACTA GCGCCACCAT GACCGAGTAC AAGCCTACCG
 2521 TGCGCCTGGC CACTCGCGAT GATGTGCCCC GCGCCGTCCG CACTCTGGCC GCCGCTTTCG
 2581 CCGACTACCC CGCTACCCGG CACACCGTGG ACCCCGACCG GCACATCGAG CGTGTGACAG
 2641 AGTTGCAGGA GCTGTTCCTG ACCCGCGTCG GGCTGGACAT CGGCAAGGTG TGGGTAGCCG
 2701 ACGACGGCGC GGCCGTGGCC GTGTGGACTA CCCCCGAGAG CGTTGAGGCC GGCGCCGTGT
 2761 TCGCCGAGAT CGGCCCCCGA ATGGCCGAGC TGAGCGGCAG CCGCCTGGCC GCCCAGCAGC
 2821 AAATGGAGGG CCTGCTTGCC CCCCATCGTC CCAAGGAGCC TGCCTGGTTT CTGGCCACTG
 2881 TAGGAGTGAG CCCCGACCAC CAGGGCAAGG GCTTGGGCAG CGCCGTCGTG TTGCCCGGCG
 2941 TAGAGGCCGC CGAACGCGCC GGTGTGCCCG CCTTTCTCGA AACAAGCGCA CCAAGAAACC
 3001 TTCCATTCTA CGAGCGCCTG GGCTTCACCG TGACCGCCGA TGTCGAGGTG CCCGAGGGAC
 3061 CTAGGACCTG GTGTATGACA CGAAAACCTG GCGCCTAATG ATCTAGAACC GGTCATGGCC
 3121 GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCGAACTA
 3181 GATGCTGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC
 3241 GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
 3301 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
 3361 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
 3421 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
 3481 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
 3541 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
 3601 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
 3661 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
 3721 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
 3781 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
 3841 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
 3901 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
 3961 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
 4021 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
 4081 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
 4141 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
 4201 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGCGGC
 4261 CGCAAATGCT AAACCACTGC AGTGGTTACC AGTGCTTGAT CAGTGAGGCA CCGATCTCAG
 4321 CGATCTGCCT ATTTCGTTCG TCCATAGTGG CCTGACTCCC CGTCGTGTAG ATCACTACGA
 4381 TTCGTGAGGG CTTACCATCA GGCCCCAGCG CAGCAATGAT GCCGCGAGAG CCGCGTTCAC
 4441 CGGCCCCCGA TTTGTCAGCA ATGAACCAGC CAGCAGGGAG GGCCGAGCGA AGAAGTGGTC
 4501 CTGCTACTTT GTCCGCCTCC ATCCAGTCTA TGAGCTGCTG TCGTGATGCT AGAGTAAGAA
 4561 GTTCGCCAGT GAGTAGTTTC CGAAGAGTTG TGGCCATTGC TACTGGCATC GTGGTATCAC
 4621 GCTCGTCGTT CGGTATGGCT TCGTTCAACT CTGGTTCCCA GCGGTCAAGC CGGGTCACAT
 4681 GATCACCCAT ATTATGAAGA AATGCAGTCA GCTCCTTAGG GCCTCCGATC GTTGTCAGAA
 4741 GTAAGTTGGC CGCGGTGTTG TCGCTCATGG TAATGGCAGC ACTACACAAT TCTCTTACCG
 4801 TCATGCCATC CGTAAGATGC TTTTCCGTGA CCGGCGAGTA CTCAACCAAG TCGTTTTGTG
 4861 AGTAGTGTAT ACGGCGACCA AGCTGCTCTT GCCCGGCGTC TATACGGGAC AACACCGCGC
 4921 CACATAGCAG TACTTTGAAA GTGCTCATCA TCGGGAATCG TTCTTCGGGG CGGAAAGACT
 4981 CAAGGATCTT GCCGCTATTG AGATCCAGTT CGATATAGCC CACTCTTGCA CCCAGTTGAT
 5041 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CGGGGTGTGC AAAAACAGGC AAGCAAAATG
 5101 CCGCAAAGAA GGGAATGAGT GCGACACGAA AATGTTGGAT GCTCATACTC GTCCTTTTTC
 5161 AATATTATTG AAGCATTTAT CAGGGTTACT AGTACGTCTC TCAAGGATAA GTAAGTAATA
 5221 TTAAGGTACG GGAGGTATTG GACAGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG
 5281 TGTGTTGGTT TTTTGTGTGA ATCGATAGTA CTAACATACG CTCTCCATCA AAACAAAACG
 5341 AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT
 5401 CTCT
//

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