LOCUS       pIRESpuro3    5157 bp     DNA  circular   SYN
DEFINITION  pIRESpuro3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="pIRESpuro3"
                     /mol_type="other DNA"
     promoter        236..852
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     promoter        863..881
                     /label="T7_promoter"
     misc_feature    1015..1310
                     /label="synth_int"
     misc_feature    1337..1900
                     /label="IRES"
     CDS             1821..2543
                     /label="ORF frame 3"
     CDS             complement(1836..2594)
                     /label="ORF frame 2"
     gene            1944..2543
                     /label="puro (variant)"
                     /gene="puro (variant)"
     terminator      2670..2898
                     /label="SV40_PA_terminator"
     misc_feature    2868..2887
                     /label="EBV_rev_primer"
     misc_feature    2921..2940
                     /label="EBV_rev_primer"
     promoter        complement(2996..3014)
                     /label="M13_reverse_primer"
     misc_feature    complement(3013..3035)
                     /label="M13_pUC_rev_primer"
     promoter        complement(3049..3078)
                     /label="lac_promoter"
     rep_origin      complement(3387..4006)
                     /label="pBR322_origin"
     gene            complement(4161..5021)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4161..5021)
                     /label="ORF frame 2"
     promoter        complement(5063..5091)
                     /label="AmpR_promoter"
ORIGIN
    1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
   61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
  121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
  181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
  241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
  301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
  361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
  421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
  481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
  541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
  601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
  661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
  721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
  781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
  841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
  901 gagctcggat cgatatctgc ggcctagcta gcgcttaagg cctgttaacc ggtcgtacgt
  961 ctccggattc gaattcggat ccgcggccgc atagataact gatccagtgt gctggaatta
 1021 attcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga
 1081 cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg
 1141 cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt
 1201 caagcttgag gtgtggcagg cttgagatct ggccatacac ttgagtgaca atgacatcca
 1261 ctttgccttt ctctccacag gtgtccactc ccaggtccaa ctgcaggtcg agcatgcatc
 1321 tagggcggcc aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
 1381 ttggaataag gccggtgtgc gtttgtctat atgtgatttt ccaccatatt gccgtctttt
 1441 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
 1501 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
 1561 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
 1621 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
 1681 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
 1741 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
 1801 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
 1861 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac
 1921 aacccacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc
 1981 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
 2041 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
 2101 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
 2161 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
 2221 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
 2281 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
 2341 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
 2401 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
 2461 ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca
 2521 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
 2581 gcgcacgacc ccatggctcc gaccgaagcc gacccgggcg gccccgccga ccccgcaccc
 2641 gcccccgagg cccaccgact ctagataact gatcataatc agccatacca catttgtaga
 2701 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
 2761 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
 2821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
 2881 actcatcaat gtatcttaac gcgtcgagtg cattctagtt gtggtttgtc caaactcatc
 2941 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
 3001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
 3061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
 3121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
 3181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
 3241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
 3301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
 3361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
 3421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
 3481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
 3541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
 3601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
 3661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
 3721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
 3781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
 3841 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
 3901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
 3961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
 4021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
 4081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
 4141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
 4201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
 4261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
 4321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
 4381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
 4441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
 4501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
 4561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
 4621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
 4681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
 4741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
 4801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
 4861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
 4921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
 4981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
 5041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
 5101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc

//