LOCUS       p3xFLAG-CMV8	4763 bp 	DNA	SYN
DEFINITION  p3xFLAG-CMV8
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="p3xFLAG-CMV8"
                     /mol_type="other DNA"
     misc_feature    complement(12..155)
                     /label="lacZ_a"
     misc_feature    126..148
                     /label="M13_pUC_fwd_primer"
     promoter        141..157
                     /label="M13_forward20_primer"
     promoter        319..895
                     /label="CMV_immearly_promoter"
     misc_feature    398..685
                     /label="CAG_enhancer"
     promoter        841..912
                     /label="CMV_promoter"
     misc_feature    852..872
                     /label="CMV_fwd_primer"
     misc_feature    896..915
                     /label="pCEP_fwd_primer"
     misc_feature    1028..1042
                     /label="EK"
     terminator      1114..1580
                     /label="hGH_PA_terminator"
     misc_feature    complement(1750..1770)
                     /label="pBABE_3_primer"
     misc_feature    complement(1756..1971)
                     /label="SV40_enhancer"
     promoter        1768..2036
                     /label="SV40_promoter"
     rep_origin      1935..2012
                     /label="SV40_origin"
     misc_feature    1997..2016
                     /label="SV40pro_F_primer"
     promoter        complement(2123..2141)
                     /label="T7_promoter"
     misc_feature    complement(2168..2190)
                     /label="M13_pUC_rev_primer"
     promoter        complement(2204..2233)
                     /label="lac_promoter"
     gene            complement(3316..4176)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3316..4176)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4218..4246)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4325..4631
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
   61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
  121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
  181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
  241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
  301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
  361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
  421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
  481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
  541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
  601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
  661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
  721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
  781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
  841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
  901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
  961 tggagctgca gttgctgact acaaagacca tgacggtgat tataaagatc atgacatcga
 1021 ttacaaggat gacgatgaca agcttgcggc cgcgaattca tcgatagatc tgatatcggt
 1081 accagtcgac tctagaggat cccgggtggc atccctgtga cccctcccca gtgcctctcc
 1141 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
 1201 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
 1261 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
 1321 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
 1381 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
 1441 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
 1501 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
 1561 ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag gacgtccaga
 1621 cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc ttgcttggca
 1681 ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt acgcggccag
 1741 cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
 1801 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
 1861 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
 1921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
 1981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
 2041 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ctcgaggaac
 2101 tgaaaaacca gaaagttaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata
 2161 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
 2221 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
 2281 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
 2341 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
 2401 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
 2461 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
 2521 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
 2581 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
 2641 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
 2701 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
 2761 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
 2821 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
 2881 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
 2941 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
 3001 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
 3061 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
 3121 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
 3181 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
 3241 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
 3301 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
 3361 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
 3421 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
 3481 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
 3541 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
 3601 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
 3661 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
 3721 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
 3781 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
 3841 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
 3901 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
 3961 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
 4021 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
 4081 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
 4141 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
 4201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
 4261 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
 4321 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
 4381 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
 4441 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
 4501 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
 4561 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
 4621 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
 4681 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
 4741 caaaatatta acgcttacaa ttt
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