LOCUS       pRS415	6021 bp 	DNA	SYN
DEFINITION  pRS415
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="pRS415"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     gene            complement(663..1757)
                     /label="LEU2 (variant)"
                     /gene="LEU2 (variant)"
     CDS             complement(663..1769)
                     /label="ORF frame 2"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     rep_origin      complement(2606..2912)
                     /label="f1_origin"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     CDS             complement(2712..3290)
                     /label="ORF frame 2"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(2945..3088)
                     /label="lacZ_a"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    3059..3081
                     /label="M13_pUC_fwd_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        3074..3090
                     /label="M13_forward20_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        3100..3118
                     /label="T7_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    3143..3159
                     /label="pBluescriptKS_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(3194..3210)
                     /label="pBluescriptSK_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(3246..3265)
                     /label="T3_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(3282..3300)
                     /label="M13_reverse_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(3299..3321)
                     /label="M13_pUC_rev_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            complement(4447..5307)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             complement(4447..5307)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5349..5377)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5439..5957
                     /label="CEN6_ARS4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
  241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
  301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
  361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
  421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
  481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
  541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
  601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
  661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
  721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
  781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
  841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
  901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
  961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
 1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
 1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
 1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
 1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
 1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
 1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
 1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
 1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
 1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
 1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
 1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
 1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
 1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
 1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
 1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
 1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
 1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
 2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
 2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
 2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
 2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
 2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
 2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
 2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
 2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
 2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
 3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
 3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
 3121 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
 3181 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
 3241 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
 3301 tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg
 3361 taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc
 3421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
 3481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
 3541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
 3601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
 3661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
 3721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
 3781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
 3841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
 3901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
 3961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
 4021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
 4081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
 4141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
 4201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
 4261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
 4321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
 4381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
 4441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
 4501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
 4561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
 4621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
 4681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
 4741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
 4801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
 4861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
 4921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
 4981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
 5041 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
 5101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
 5161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
 5221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
 5281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
 5341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
 5401 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat
 5461 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
 5521 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
 5581 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
 5641 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
 5701 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
 5761 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
 5821 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
 5881 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
 5941 aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
 6001 gtatcacgag gccctttcgt c
//